1. Immunocytological detection of AAF-DNA adducts in HeLa cell nuclei.
- Author
-
Sage E, Gabelman N, Mendez F, and Bases R
- Subjects
- Antibody Specificity, Dose-Response Relationship, Drug, Fluorescent Antibody Technique, HeLa Cells immunology, HeLa Cells ultrastructure, Humans, Time Factors, 2-Acetylaminofluorene metabolism, Antibodies, Antinuclear analysis, Cell Nucleus immunology, DNA immunology
- Abstract
Acetylaminofluorene-DNA adducts (AAF-DNA) were detected in the nuclei of HeLa cells exposed to N-acetoxy-2-acetylaminofluorene (N-Ac-AAF), using an immunocytological technique and specific antibodies directed against AAF modified DNA. The proportion of cells exhibiting specific nuclear immunoreactivity was dose-dependent. The time course of disappearance of adduct specific nuclear immunoreactivity was compared with removal of N-(deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-C8-AAF) and other adducts.
- Published
- 1981
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