1. Lebectin increases N-cadherin-mediated adhesion through PI3K/AKT pathway
- Author
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Mohamed El Ayeb, Naziha Marrakchi, Carole Siret, Frédéric André, Sameh Sarray, José Luis, Maxime Lehmann, Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP), Faculté des Sciences, Faculté des Sciences Mathématiques, Physiques et Naturelles de Tunis (FST), Université de Tunis El Manar (UTM)-Université de Tunis El Manar (UTM), Centre de Recherches en Oncologie biologique et Oncopharmacologie (CRO2), Aix Marseille Université (AMU)- Hôpital de la Timone [CHU - APHM] (TIMONE)-Institut National de la Santé et de la Recherche Médicale (INSERM), Faculté de Médecine de Tunis, and Université de Tunis El Manar (UTM)
- Subjects
MESH: Signal Transduction ,Cancer Research ,MESH: Cell Line, Tumor ,Blotting, Western ,Integrin ,Viper Venoms ,Biology ,MESH: Cadherins ,MESH: Cell Adhesion ,Phosphatidylinositol 3-Kinases ,03 medical and health sciences ,0302 clinical medicine ,Cell–cell interaction ,Cell Movement ,Cell Line, Tumor ,Cell Adhesion ,Humans ,Immunoprecipitation ,MESH: Blotting, Western ,Lectins, C-Type ,CD93 ,Cell adhesion ,MESH: Cell Movement ,030304 developmental biology ,0303 health sciences ,MESH: Humans ,MESH: Proto-Oncogene Proteins c-akt ,MESH: Immunoprecipitation ,Cell adhesion molecule ,Cadherin ,MESH: Immunohistochemistry ,Cadherins ,Intercellular adhesion molecule ,Immunohistochemistry ,3. Good health ,Cell biology ,Oncology ,MESH: Phosphatidylinositol 3-Kinases ,030220 oncology & carcinogenesis ,biology.protein ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,Neural cell adhesion molecule ,MESH: Viper Venoms ,Proto-Oncogene Proteins c-akt ,MESH: Lectins, C-Type ,Signal Transduction - Abstract
International audience; Cell adhesion molecules, including cadherins and integrins, play an essential role during tumor progression and represent potential targets for the development of new therapeutic agents. We previously showed that lebectin, a C-type lectin protein (CLP) issued from Macrovipera lebectina snake venom, inhibits integrin-mediated migration of IGR39 melanoma cells. Here we assessed whether lebectin modulates cell-cell adhesion. We demonstrated that lebectin promotes N-cadherin/catenin complex reorganization at cell-cell contacts, inducing a strengthening of intercellular adhesion. This reorganization is associated to phosphorylation of beta-catenin on tyrosine 142 residue. Interestingly, lebectin acts on N-cadherin-mediated cell-cell contacts through PI3K/Akt pathway. This effect could contribute to the blockage of tumor cell migration previously observed.
- Published
- 2009