1. Diverse roles of hepatocyte growth factor activator inhibitor type 1 (HAI-1) in the growth of glioblastoma cells in vivo
- Author
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Tsuyoshi Fukushima, Shunro Uchinokura, Shinichiro Wakisaka, Shinichi Nakano, Hiroaki Kataoka, Yutaka Akiyama, Hiroshi Itoh, Ryouichi Hamasuna, and Shiro Miyata
- Subjects
Male ,Cancer Research ,animal structures ,Proteinase Inhibitory Proteins, Secretory ,Biology ,Mice ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Mice, Inbred BALB C ,Membrane Glycoproteins ,Expression vector ,virus diseases ,HEPATOCYTE GROWTH FACTOR ACTIVATOR INHIBITOR ,Transfection ,medicine.disease ,Protease inhibitor (biology) ,In vitro ,Oncology ,Cell culture ,Cancer research ,Glioblastoma ,medicine.drug - Abstract
Hepatocyte growth factor activator inhibitor type-1 (HAI-1) is an integral-membrane proteinase inhibitor. In this study, we examined the effects of HAI-1 on human glioblastoma cells. Two glioblastoma cell lines (YKG-1, U251) were stably transfected with expression plasmid harboring mature membrane-form or truncated secreted-form HAI-1. Culture characteristics were not altered by the expression of HAI-1, whereas in vitro invasiveness of U251 was suppressed. On the other hand, the expression of membrane-form HAI-1 resulted in significantly enhanced tumorigenicity of both cell lines in vivo. In contrast, secreted-form HAI-1 did not promote the tumorigenicity. These results suggest that HAI-1 may play complex roles in progression of glioblastoma cells, and membrane-form HAI-1 may mediate an undefined important signaling in the cells.
- Published
- 2005
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