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Xiao-Yu Su,1 Qian Zhao,1 Jin-Ming Ke,2 De-Hong Wu,1 Xin Zhu,1 Jiang Lin,1 Zhao-Qun Deng1 1Laboratory Center, The Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu, People’s Republic of China; 2Faculty of Forestry, Zhejiang A&F University, Hangzhou, Zhejiang, People’s Republic of ChinaCorrespondence: Zhao-Qun DengLaboratory Center, The Affiliated People’s Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang, Jiangsu 212002, People’s Republic of ChinaFax +86 51185234387Email zqdeng2002@163.comPurpose: Our research aimed to investigate the expression level of circ_ 0002232, which is transcribed from PTEN, and find out the association of circ_0002232/miR-92a-3p/PTEN network in acute myeloid leukemia (AML).Methods: Circ_0002232 expression in 115 AML patients and 48 controls was detected by using real-time quantitative PCR. The diagnostic value of circ_0002232 expression was evaluated by receiver operating characteristic curve. Kaplan–Meier curves were used to analyse the impact of circ_0002232 for overall survival. Associated network of circ_0002232 was predicted by using interaction prediction websites.Results: Compared with controls, circ_0002232 was notably low-expressed in AML (P< 0.001). According to the result of receiver operating characteristic curve, circ_0002232 expression could distinguish AML patients from controls (P< 0.001). There were significant differences in patients’ age (P=0.004), FAB classifications (P=0.036), white blood cell count (P=0.041) and platelet count (P=0.021) between low-expressed circ_0002232 group and high-expressed circ_0002232 group. Moreover, there was a positive correlation between circ_0002232 expression and patients’ age (Pearson r=0.256, P=0.0057). Interestingly, we found that patients in low-expressed circ_0002232 group had better overall survival both in whole AML (P=0.030) and non-APL AML (P=0.014). Remarkably, the expression of circ_0002232 was positively correlated with PTEN (Spearman r=0.678, P< 0.001). Furthermore, there was a negative correlation in AML between circ_0002232 and miR-92a-3p (Spearman r=− 0.301, P=0.016), miR-92a-3p and PTEN (Spearman r=− 0.324, P=0.034). Interaction prediction websites revealed that circ_0002232 might affect the expression of PTEN and the process of AML through sponging miR-92a-3p.Conclusion: Circ_0002232, one of the circRNAs transcribed from PTEN, was remarkably down-regulated in AML and could act as a promising biomarker for the diagnosis of AML. In addition, there might be a potential association network of circ_0002232/miR-92a-3p/PTEN in AML.Keywords: circular RNAs, circ_0002232, miR-92a-3p, PTEN, acute myeloid leukemia

Lan Yang,1,2,* Jing-Dong Zhou,2,3,* Ting-Juan Zhang,2,3 Ji-Chun Ma,1,2 Gao-Fei Xiao,1,2 Qin Chen,1,2 Zhao-Qun Deng,1,2 Jiang Lin,1,2 Jun Qian,2,3 Dong-Ming Yao1,2 1Laboratory Center, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu, People’s Republic of China; 2The Key Laboratory of Precision Diagnosis and Treatment of Zhenjiang City, Zhenjiang, Jiangsu, People’s Republic of China; 3Department of Hematology, Affiliated People’s Hospital of Jiangsu University, Zhenjiang, Jiangsu, People’s Republic of China *These authors contributed equally to this work Background and purpose: Abundant studies have shown that lncRNA PANDAR plays an oncogenic role in human solid tumors. Although abnormal expression of PANDAR has been well investigated in solid tumors, it was rarely studied in hematologic diseases. Hence, the aim of this study was to determine the PANDAR expression level and its clinical significance in patients with acute myeloid leukemia (AML).Materials and methods: For detecting the expression level of PANDAR in 119 AML patients and 26 controls, real-time quantitative PCR was used in this study. The prognostic values were evaluated by using Kaplan–Meier analysis, Cox regression analyses, and logistic regression analysis.Results: PANDAR was significantly overexpressed in AML and might be a promising biomarker which could distinguish AML from normal samples (P