1. Recombinant human stem cell factor stimulates growth of a human glioblastoma cell line expressing c-kit protooncogene.
- Author
-
Berdel WE, de Vos S, Maurer J, Oberberg D, von Marschall Z, Schroeder JK, Li J, Ludwig WD, Kreuser ED, and Thiel E
- Subjects
- Cell Division drug effects, Dose-Response Relationship, Drug, Flow Cytometry, Glioma genetics, Humans, Proto-Oncogene Proteins analysis, Proto-Oncogene Proteins c-kit, Proto-Oncogenes, Recombinant Proteins pharmacology, Stem Cell Factor, Tumor Cells, Cultured, Tumor Stem Cell Assay, Glioma pathology, Hematopoietic Cell Growth Factors pharmacology
- Abstract
The growth of a panel of eight different human glioblastoma cell lines was examined in a human tumor cloning assay in agar, a tritiated thymidine uptake assay, and by counting cell numbers, in cultures performed in the absence or presence of increasing concentrations (1 to 100 ng/ml) of recombinant human stem cell factor (SCF). Growth of 7 of 8 cell lines was not significantly and reproducibly affected by recombinant human SCF. However, growth of the CRL 1620 cell line could be stimulated up to 5-fold by the cytokine. In contrast to the other cell lines investigated, CRL 1620 expressed the c-kit protooncogene assessed on the mRNA and protein level. Furthermore, SCF-induced proliferation of CRL 1620 cells was sensitive to the tyrosine kinase inhibitor erbstatin. Our data suggest that SCF can be operative in growth modulation of malignant cells outside the hematopoietic system, and this finding should be further studied for its possible clinical implications.
- Published
- 1992