1. Functional Effects of let-7g Expression in Colon Cancer Metastasis.
- Author
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Chang, Che-Mai, Wong, Henry Sung-Ching, Huang, Chien-Yu, Hsu, Wen-Li, Maio, Zhi-Feng, Chiu, Siou-Jin, Tsai, Yao-Ting, Chen, Ben-Kuen, Wan, Yu-Jui Yvonne, Wang, Jaw-Yuan, and Chang, Wei-Chiao
- Subjects
ANIMAL experimentation ,CELL physiology ,COLON tumors ,GENE expression ,GENOMES ,METASTASIS ,MICE ,RNA - Abstract
MicroRNA regulation is crucial for gene expression and cell functions. It has been linked to tumorigenesis, development and metastasis in colorectal cancer (CRC). Recently, the let-7 family has been identified as a tumor suppressor in different types of cancers. However, the function of the let-7 family in CRC metastasis has not been fully investigated. Here, we focused on analyzing the role of let-7g in CRC. The Cancer Genome Atlas (TCGA) genomic datasets of CRC and detailed data from a Taiwanese CRC cohort were applied to study the expression pattern of let-7g. In addition, in vitro as well as in vivo studies have been performed to uncover the effects of let-7g on CRC. We found that the expression of let-7g was significantly lower in CRC specimens. Our results further supported the inhibitory effects of let-7g on CRC cell migration, invasion and extracellular calcium influx through store-operated calcium channels. We report a critical role for let-7g in the pathogenesis of CRC and suggest let-7g as a potential therapeutic target for CRC treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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