1. Chitosan-gentamicin conjugate attenuates heat stress-induced intestinal barrier injury via the TLR4/STAT6/MYLK signaling pathway: In vitro and in vivo studies.
- Author
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Niu, Xueting, Hu, Canying, Chen, Shengwei, Wen, Jiaying, Liu, Xiaoxi, Yong, Yanhong, Yu, Zhichao, Ma, Xingbin, Li, Chengpeng, Warda, Mohamad, Abd El-Aty, A.M., Gooneratne, Ravi, and Ju, Xianghong
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INTESTINAL injuries , *CELLULAR signal transduction , *OCCLUDINS , *IN vivo studies , *CARRIER proteins , *IN vitro studies - Abstract
Heat stress (HS) has a negative impact on animal health. A modified chitosan-gentamicin conjugate (CS-GT) was prepared to investigate its potential protective effects and mechanism of action on heat stress-induced intestinal mucosa injury in IPEC-J2 cells and mouse 3D intestinal organs in a mouse model. CS-GT significantly (P < 0.01) reversed the decline in transmembrane resistance and increased the FITC-dextran permeability of the IPEC-J2 monolayer fusion epithelium caused by heat stress. Heat stress decreased the expression of the tight binding proteins occludin, claudin1, and claudin2. However, pretreatment with CS-GT significantly increased (P < 0.01) the expression of these tight binding proteins. Mechanistically, CS-GT inhibited the activation of the TLR4/STAT6/MYLK signaling pathway induced by heat stress. Molecular docking showed that CS-GT can bind effectively with TLR4. In conclusion, CS-GT alleviates heat stress-induced intestinal mucosal damage both in vitro and in vivo. This effect is mediated, at least partly, by the inhibition of the TLR4/STAT6/MYLK signaling pathway and upregulation of tight junction proteins. These findings suggest that CS-GT may have therapeutic potential in the prevention and treatment of heat stress-related intestinal injury. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2023
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