1. Quantitative analysis of anions in glycosaminoglycans and application in heparin stability studies.
- Author
-
Liu L, Linhardt RJ, and Zhang Z
- Subjects
- Anions analysis, Carbohydrate Conformation, Chlorides analysis, Chromatography, High Pressure Liquid, Drug Stability, Heparin chemistry, Hydrogen-Ion Concentration, Hydrolysis, Sensitivity and Specificity, Temperature, Acetates analysis, Glycosaminoglycans chemistry, Sulfates analysis
- Abstract
The sulfo groups of glycosaminoglycans contribute to their high charge densities, and are critical for the role they play in various physiological and pathophysiological processes. Unfortunately, the sulfo groups can be hydrolyzed to inorganic sulfate. Thus, it is important to monitor the presence of these sulfo groups. In addition, free anions, including chloride, sulfate and acetate, are often present in glycosaminoglycans as a result of multiple purification steps, and their presence also needs to be monitored. In this report, ion chromatography with conductivity detection is used to analyze the anions present in glycosaminoglycans, including heparin, heparan sulfate, chondroitin sulfate and dermatan sulfate. This method allows quantitation over a wide range of concentrations, affording a limit of quantitation of 0.1 ppm and a limit of detection of 0.05 ppm for most anions of interest. The stability of heparin was also studied, providing data on the formation of both sulfate and acetate anions., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2014
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