1. Dual-drug delivery of Ag-chitosan nanoparticles and phenytoin via core-shell PVA/PCL electrospun nanofibers.
- Author
-
Mohamady Hussein MA, Guler E, Rayaman E, Cam ME, Sahin A, Grinholc M, Sezgin Mansuroglu D, Sahin YM, Gunduz O, Muhammed M, El-Sherbiny IM, and Megahed M
- Subjects
- Anti-Bacterial Agents pharmacology, Calorimetry, Differential Scanning methods, Cell Proliferation drug effects, Chitosan pharmacology, Escherichia coli drug effects, Microscopy, Electron, Scanning methods, Phenytoin pharmacology, Polyesters chemistry, Polyvinyl Alcohol chemistry, Silver pharmacology, Spectroscopy, Fourier Transform Infrared methods, Staphylococcus aureus drug effects, Wound Healing drug effects, Chitosan chemistry, Drug Delivery Systems methods, Nanofibers chemistry, Nanoparticles chemistry, Phenytoin chemistry, Silver chemistry
- Abstract
Dual-drug delivery systems were constructed through coaxial techniques, which were convenient for the model drugs used the present work. This study aimed to fabricate core-shell electrospun nanofibrous membranes displaying simultaneous cell proliferation and antibacterial activity. For that purpose, phenytoin (Ph), a well-known proliferative agent, was loaded into a polycaprolactone (PCL) shell membrane, and as-prepared silver-chitosan nanoparticles (Ag-CS NPs), as biocidal agents, were embedded in a polyvinyl alcohol (PVA) core layer. The morphology, chemical composition, mechanical and thermal properties of the nanofibrous membranes were characterized by FESEM/STEM, FTIR and DSC. The coaxial PVA-Ag CS NPs/PCL-Ph nanofibers (NFs) showed more controlled Ph release than PVA/PCL-Ph NFs. There was notable improvement in the morphology, thermal, mechanical, antibacterial properties and cytobiocompatibility of the fibers upon incorporation of Ph and Ag-CS NPs. The proposed core-shell PVA/PCL NFs represent promising scaffolds for tissue regeneration and wound healing by the effective dual delivery of phenytoin and Ag-CS NPs., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF