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In this study, we prepared Rhizoma Phragmites derived oligosaccharides (ROs) by hydrolysis with hydrogen peroxide (H2O2). The ROs yield was affected by reaction time, temperature, and H2O2 concentration. Too long reaction time and too high temperature decreased the ROs yield. Maximum ROs yield (11.26%) was obtained at reaction time 4h, temperature 75 °C, and H2O2 concentration 3.5% (v/v). The oligosaccharides sample contained 93.16% sugar, of which the average degree was approximately 11, was water-soluble, and showed white. The ROs had the highest antibacterial activity against Staphylococcus aureus, followed by Bacillus subtilis and Escherichia coli at the concentration of 100 μg/mL., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

In this study, the cellulase-assisted extraction and antioxidant activity of the polysaccharides from Rhizoma imperata were investigated. To improve the yield of R. Imperata polysaccharides (RPs), the extraction conditions were optimized as follows: time, 69.48 min; temperature, 45.36°C; pH, 4.58; cellulase amount, 1,200 U/g. Under these optimum conditions, the yield of RPs reached 0.67% (w/w), and was higher than that of the traditionally aqueous extraction method. The sugar content in the RPs product reached up to 93.25% (w/w). The RPs product has high antioxidant activity including hydroxyl radical scavenging activity and 2,2-diphenyl-β-picrylhydrazyl radical scavenging activity at the concentration of 100mg/mL., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

In this study, the Lycium barbarum oligosaccharides (LBO) were prepared by hydrolysis using hydrogen peroxide (H₂O₂). The yield of the LBO was monitored during the hydrolysis process. The hydrolysis conditions were optimized as follows: time, 4h; temperature, 70 °C; and H₂O₂ concentration, 2.5% (v/v). The hydrolysates were filtered, concentrated to ∼20% (w/v), precipitated with 6 volumes of absolute ethanol, freeze-dried, and ground to yield a water soluble and white powder. The sugar content of the product was 95.8%, and the yield was 21.05% (w/w), respectively. The LBO show higher hydroxyl radical scavenging activity (86.46%) than Vc (40.96) at the concentration of 100 μg/mL., (Copyright © 2013 Elsevier Ltd. All rights reserved.)