1. Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510.
- Author
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Zhang Z, Yu G, Guan H, Zhao X, Du Y, and Jiang X
- Subjects
- Chromatography, High Pressure Liquid, Magnetic Resonance Spectroscopy, Oligosaccharides isolation & purification, Spectrometry, Mass, Electrospray Ionization, Alginates chemistry, Bacteria enzymology, Glucuronic Acid chemistry, Hexuronic Acids chemistry, Oligosaccharides chemistry, Oligosaccharides metabolism, Polysaccharide-Lyases metabolism
- Abstract
Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed., (Copyright 2004 Elsevier Ltd.)
- Published
- 2004
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