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Start Over Author "Vinogradov, A." Journal carbohydrate research
475 results on '"Vinogradov, A."'

41. Structural analysis of the core oligosaccharides from Fusobacterium nucleatum lipopolysaccharides

Author

Evgeny Vinogradov, Andrew D. Cox, Perry Fleming, and Frank St. Michael

Subjects
Lipopolysaccharides, Magnetic Resonance Spectroscopy, Lipopolysaccharide, Heptose, Oligosaccharides, 010402 general chemistry, 01 natural sciences, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, Residue (chemistry), Carbohydrate Conformation, structure, chemistry.chemical_classification, biology, Strain (chemistry), Fusobacterium nucleatum, 010405 organic chemistry, Organic Chemistry, lipopolysaccharide, General Medicine, biology.organism_classification, NMR, 0104 chemical sciences, stomatognathic diseases, chemistry, Aeromonas, Acid hydrolysis, Bacteria
Abstract

Fusobacterium nucleatum is a gram-negative bacterium, part of the normal human microflora. It is associated with various health complications, including periodontitis and colorectal cancer. Its surface is covered with lipopolysaccharide, which interacts with the immune system and can be involved in various processes in health and disease conditions. Here we present the results of structural analysis of core oligosaccharides from the lipopolysaccharides of several strains of F. nucleatum. Pure compounds were isolated using mild acid hydrolysis or alkaline deacylation of the lipopolysaccharides and analyzed by NMR spectroscopy, mass-spectrometry and chemical methods. All cores analyzed had a common octasaccharide region, including five heptose residues and a non-phosphorylated 3-deoxy-d-manno-oct-2-ulosonic acid residue. The common region is substituted with different additional components specific for each strain. By structure type the F. nucleatum core is similar to that produced by Aeromonas.

Published
2020

42. Structural analysis of the core and polysaccharide from the lipopolysaccharide produced by Chromobacterium violaceum strain ATCC 12472 (NCTC 9757)

Author

Otto Holst, Buko Lindner, and Evgeny Vinogradov

Subjects
Lipopolysaccharides, Lipopolysaccharide, Stereochemistry, 010402 general chemistry, Polysaccharide, 01 natural sciences, Biochemistry, Analytical Chemistry, chemistry.chemical_compound, structure, Strain atcc, chemistry.chemical_classification, Strain (chemistry), biology, 010405 organic chemistry, Chemistry, Chromobacterium, Organic Chemistry, General Medicine, Hydrogen-Ion Concentration, biology.organism_classification, NMR, nonulosonic acid, 0104 chemical sciences, Carbohydrate Sequence, Chromobacterium violaceum, Chromobacterium violaceum: LPS
Abstract

The structure of the polysaccharide O-chain of the lipopolysaccharide isolated from the sequenced strain Chromobacterium violaceum ATCC 12472 (NCTC 9757) was investigated by chemical and NMR analyses, and concluded to be -4-α-Leg5Ac7Ala-4-β-d-ManNAlaA3OAc-3-α-d-GlcNAc-where Leg5Ac7Ala indicates 5-acetamido-7-alanylamido-3,5,7,9-tetradeoxy-d-glycero-d-galacto-non-2-ulopyranosonic acid and ManNAlaA3OAc 3-O-acetyl-2-alanylamido-2-deoxymannopyranuronic acid. The structure of the core with one repeating unit of the polysaccharide attached was also analyzed, and it was found that the O-chain polysaccharide is linked to the core via β-GlcpNAc, as opposite to α-GlcpNAc inside the O-chain.

Published
2020

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