1. Mapping the whole human genome by fingerprinting yeast artificial chromosomes.
- Author
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Bellanné-Chantelot C, Lacroix B, Ougen P, Billault A, Beaufils S, Bertrand S, Georges I, Glibert F, Gros I, and Lucotte G
- Subjects
- Base Sequence, Chromosomes, Fungal, DNA Probes, Gene Library, Humans, Molecular Sequence Data, Repetitive Sequences, Nucleic Acid, Restriction Mapping, Chromosome Mapping methods, Genome, Human
- Abstract
Physical mapping of the human genome has until now been envisioned through single chromosome strategies. We demonstrate that by using large insert yeast artificial chromosomes (YACs) a whole genome approach becomes feasible. YACs (22,000) of 810 kb mean size (5 genome equivalents) have been fingerprinted to obtain individual patterns of restriction fragments detected by a LINE-1 (L1) probe. More than 1000 contigs were assembled. Ten randomly chosen contigs were validated by metaphase chromosome fluorescence in situ hybridization, as well as by analyzing the inter-Alu PCR patterns of their constituent YACs. We estimate that 15% to 20% of the human genome, mainly the L1-rich regions, is already covered with contigs larger than 3 Mb.
- Published
- 1992
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