1. Pheromone-Dependent Destruction of the Tec1 Transcription Factor Is Required for MAP Kinase Signaling Specificity in Yeast
- Author
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John R. Yates, Greg T. Cantin, Hiten D. Madhani, Marie Z. Bao, and Monica A. Schwartz
- Subjects
Threonine ,MAPK/ERK pathway ,Saccharomyces cerevisiae Proteins ,MAP Kinase Signaling System ,Molecular Sequence Data ,Cell Cycle Proteins ,Saccharomyces cerevisiae ,F-box protein ,Anaphase-Promoting Complex-Cyclosome ,Pheromones ,General Biochemistry, Genetics and Molecular Biology ,Substrate Specificity ,03 medical and health sciences ,0302 clinical medicine ,Amino Acid Sequence ,Phosphorylation ,Kinase activity ,Transcription factor ,030304 developmental biology ,0303 health sciences ,SKP Cullin F-Box Protein Ligases ,biology ,Ubiquitin ,Biochemistry, Genetics and Molecular Biology(all) ,F-Box Proteins ,Ubiquitin-Protein Ligase Complexes ,Cullin Proteins ,Molecular biology ,Cell biology ,DNA-Binding Proteins ,Crosstalk (biology) ,Mutation ,Fus3 ,biology.protein ,Mitogen-Activated Protein Kinases ,030217 neurology & neurosurgery ,Cullin ,Protein Binding ,Transcription Factors - Abstract
The yeast MAPK pathways required for mating versus filamentous growth share multiple components yet specify distinct programs. The mating-specific MAPK, Fus3, prevents crosstalk between the two pathways by unknown mechanisms. Here we show that pheromone signaling induces Fus3-dependent degradation of Tec1, the transcription factor specific to the filamentation pathway. Degradation requires Fus3 kinase activity and a MAPK phosphorylation site in Tec1 at threonine 273. Fus3 associates with Tec1 in unstimulated cells, and active Fus3 phosphorylates Tec1 on T273 in vitro. Destruction of Tec1 requires the F box protein Dia2 (Digs-into-agar-2), and Cdc53, the Cullin of SCF (Skp1-Cdc53-F box) ubiquitin ligases. Notably, mutation of the phosphoacceptor site in Tec1, deletion of FUS3, or deletion of DIA2 results in a loss of signaling specificity such that pheromone pathway signaling erroneously activates filamentation pathway gene expression and invasive growth. Signal-induced destruction of a transcription factor for a competing pathway provides a mechanism for signaling specificity.
- Published
- 2004
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