1. Genome Sequencing and Analysis of the Tasmanian Devil and Its Transmissible Cancer
- Author
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Graham R. Bignell, Thomas R. Connor, Ludmil B. Alexandrov, Lisa Murray, Sean Humphray, Bee Ling Ng, Geoffrey Paul Smith, Wendy S.W. Wong, Zemin Ning, Michael R. Stratton, Shujun Luo, Zhi-Ping Feng, Anthony J. Cox, Peter J. Campbell, Philip Tedder, Albert J. Vilella, Niall Anthony Gormley, David J. McBride, Simon R. Harris, Keiran Raine, Bronwen Aken, Elizabeth P. Murchison, R. Keira Cheetham, Carolyn Tregidgo, Matthew M. Hims, P. Andrew Futreal, Sergii Ivakhno, Dirk J. Evers, Markus J. Bauer, Isabelle Rasolonjatovo, Yong Gu, Zoya Kingsbury, Simon D. M. White, William Cheng, Fengtang Yang, Anne-Maree Pearse, Amber E. Alsop, Beiyuan Fu, Gregory M. Woods, Gary P. Schroth, Stephen M. J. Searle, Kevin Hall, Mark Kowarsky, David R. Bentley, David C. Wedge, Irina Khrebtukova, Ole Schulz-Trieglaff, Jennifer Becq, Caitlin Stewart, Nigel P. Carter, Richard Shaw, John Marshall, Alexandre Kreiss, Zhihao Ding, Anthony T. Papenfuss, and Russell J. Grocock
- Subjects
Male ,0106 biological sciences ,Lineage (genetic) ,Molecular Sequence Data ,Devil facial tumour disease ,Genomics ,Biology ,010603 evolutionary biology ,01 natural sciences ,Somatic evolution in cancer ,Genome ,Article ,Genomic Instability ,Tasmania ,General Biochemistry, Genetics and Molecular Biology ,Clonal Evolution ,03 medical and health sciences ,Tasmanian devil ,medicine ,Animals ,030304 developmental biology ,Marsupial ,Genetics ,0303 health sciences ,Biochemistry, Genetics and Molecular Biology(all) ,Endangered Species ,medicine.disease ,biology.organism_classification ,Marsupialia ,Sarcophilus ,Mutation ,Female ,Facial Neoplasms ,Genome-Wide Association Study - Abstract
Summary The Tasmanian devil (Sarcophilus harrisii), the largest marsupial carnivore, is endangered due to a transmissible facial cancer spread by direct transfer of living cancer cells through biting. Here we describe the sequencing, assembly, and annotation of the Tasmanian devil genome and whole-genome sequences for two geographically distant subclones of the cancer. Genomic analysis suggests that the cancer first arose from a female Tasmanian devil and that the clone has subsequently genetically diverged during its spread across Tasmania. The devil cancer genome contains more than 17,000 somatic base substitution mutations and bears the imprint of a distinct mutational process. Genotyping of somatic mutations in 104 geographically and temporally distributed Tasmanian devil tumors reveals the pattern of evolution and spread of this parasitic clonal lineage, with evidence of a selective sweep in one geographical area and persistence of parallel lineages in other populations. PaperClip, Graphical Abstract Highlights ► Whole-genome sequences of the Tasmanian devil and two distant cancer subclones ► The Tasmanian devil cancer lineage originated recently in a female devil ► The devil cancer genome is relatively stable despite ongoing evolution ► Clonal divergence and geographic spread elucidated through patterns of mutation, Whole-genome sequences of the Tasmanian devil and two devil cancer subclones suggest that the cancer first arose from a female devil and that the clone has subsequently genetically diverged during its spread across Tasmania.
- Published
- 2012
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