1. In vitro cytotoxicity of chrysotile asbestos to human pulmonary alveolar macrophages is decreased by organosilane coating and surfactant
- Author
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M. V. Marshall, David L. Busbee, Lawrence Ec, Feuerbacher Dg, Myles L. Mace, A. C. Griffin, Morrison Dg, and Theodore L. McLemore
- Subjects
Adult ,Male ,Pathology ,medicine.medical_specialty ,food.ingredient ,Asbestos, Serpentine ,Health, Toxicology and Mutagenesis ,In vitro cytotoxicity ,Pharmacology toxicology ,In Vitro Techniques ,Toxicology ,medicine.disease_cause ,Lecithin ,Asbestos ,Surface-Active Agents ,food ,Pulmonary surfactant ,Chrysotile ,medicine ,Humans ,Cytotoxic T cell ,Mutagenicity Tests ,Chemistry ,Macrophages ,technology, industry, and agriculture ,Cell Biology ,respiratory system ,V79 cells ,Molecular biology ,Pulmonary Alveoli ,Female - Abstract
Human pulmonary alveolar macrophages were used to quantitate the cytotoxic effect of surface-altered chrysotile asbestos. Little difference was observed in mortality between chrysotile asbestos that was surface-treated to a 42% extent by a hydrophobic organosilane or untreated chrysotile. Little or no effect on mortality was observed when human pulmonary alveolar macrophages were cultured with untreated chrysotile or acid-leached asbestos in the presence of 10 mM dipalmitoyl lecithin. However, when human pulmonary alveolar macrophages were cultured with a hydrophobically-treated (to a 42% or 95% extent) chrysotile asbestos in the presence of 10 mM dipalmitoyl lecithin, a statistically significant decrease in mortality was observed compared to untreated chrysotile. No mutagenic activity was observed when V79 cells were cultured with acid-leached, or 42% hydrophobically-treated chrysotile asbestos, even when human pulmonary alveolar macrophages were included as an activation source. The 95% hydrophobically-treated and acid-leached chrysotile also exhibited decreased binding of benzo[a]pyrene compared to untreated chrysotile asbestos.
- Published
- 1986
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