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Start Over Author "Danielle Courtney" Journal cell biology international
2 results on '"Danielle Courtney"'

1. Differential protein abundance of a basolateral MCT1 transporter in the human gastrointestinal tract

Author

Stefanie Riveros‐Beltran, Naomi Fearon, Alison McGrane, Gavin Stewart, Elizabeth P. Ryan, Hashemeyah Al‐mosauwi, C Walpole, Eugene M. Dempsey, Alan W. Baird, Danielle Courtney, and Desmond C. Winter

Subjects
0301 basic medicine, Gastrointestinal tract, Human gastrointestinal tract, Sigmoid colon, Ileum, Cell Biology, General Medicine, Butyrate, Biology, Molecular biology, Descending colon, 03 medical and health sciences, 030104 developmental biology, medicine.anatomical_structure, Biochemistry, Symporter, medicine, Ascending colon
Abstract

Bacterially derived short chain fatty acids (SCFAs), such as butyrate, are vital in maintaining the symbiotic relationship that exists between humans and their gastrointestinal microbial populations. A key step in this process is the transport of SCFAs across colonic epithelial cells via MCT1 transporters. This study investigated MCT1 protein abundance in various human intestinal tissues. Initial RT-PCR analysis confirmed the expected MCT1 RNA expression pattern of colon > small intestine > stomach. Using surgical resection samples, immunoblot analysis detected higher abundance of a 45 kDa MCT1 protein in colonic tissue compared to ileum tissue (P

Published
2016
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2. Differential protein abundance of a basolateral MCT1 transporter in the human gastrointestinal tract

Author

Hashemeya, Al-Mosauwi, Elizabeth, Ryan, Alison, McGrane, Stefanie, Riveros-Beltran, Caragh, Walpole, Eugene, Dempsey, Danielle, Courtney, Naomi, Fearon, Desmond, Winter, Alan, Baird, and Gavin, Stewart

Subjects
Gastrointestinal Tract, Monocarboxylic Acid Transporters, Gene Expression Regulation, Symporters, Colon, Ileum, Fluorescent Antibody Technique, Humans, Peptide Transporter 1
Abstract

Bacterially derived short chain fatty acids (SCFAs), such as butyrate, are vital in maintaining the symbiotic relationship that exists between humans and their gastrointestinal microbial populations. A key step in this process is the transport of SCFAs across colonic epithelial cells via MCT1 transporters. This study investigated MCT1 protein abundance in various human intestinal tissues. Initial RT-PCR analysis confirmed the expected MCT1 RNA expression pattern of colon small intestine stomach. Using surgical resection samples, immunoblot analysis detected higher abundance of a 45 kDa MCT1 protein in colonic tissue compared to ileum tissue (P 0.001, N = 4, unpaired t-test). Importantly, MCT1 abundance was found to be significantly lower in sigmoid colon compared to ascending colon (P 0.01, N = 8-11, ANOVA). Finally, immunolocalization studies confirmed MCT1 to be abundant in the basolateral membranes of surface epithelial cells of the ascending, transverse, and descending colon, but significantly less prevalent in the sigmoid colon (P 0.05, N = 5-21, ANOVA). In conclusion, these data confirm that basolateral MCT1 protein abundance is correlated to levels of bacterially derived SCFAs along the human gastrointestinal tract. These findings highlight the importance of precise tissue location in studies comparing colonic MCT1 abundance between normal and diseased states.

Published
2015

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