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Start Over Author "Becchetti, E." Journal cell biology international reports
5 results on '"Becchetti, E."'

4. Age related and lectin influenced changes of exoglycosidases activity in cultured chick embryonic skin fibroblasts.

Author

Bodo M, Pezzetti F, Becchetti E, Orlacchio A, Evangelisti R, and Carinci P

Subjects
Animals, Cells, Cultured, Chick Embryo, Concanavalin A pharmacology, Fibroblasts, Glycosaminoglycans metabolism, Peanut Agglutinin, Phosphoric Diester Hydrolases metabolism, Skin enzymology, Wheat Germ Agglutinins pharmacology, beta-N-Acetyl-Galactosaminidase, Acetylglucosaminidase metabolism, Galactosidases metabolism, Hexosaminidases metabolism, Lectins pharmacology, Plant Lectins, Skin cytology, Soybean Proteins, beta-Galactosidase metabolism
Abstract

beta-N-acetyl-D-glucosaminidase, beta-N-acetyl-galactosaminidase and a beta-D-galactosidase activity was determined in untreated and lectins (ConA, PNA, SBA and WGA) treated chick embryonic skin fibroblasts at two incubation stages. Activity of all three glycosidases increased between 7 and 14 incubation days. ConA and WGA affected the levels of enzymatic activity; while SBA and PNA were uneffective. We discuss these findings in relation to a possible role of glycosidases in controlling mesenchymal GAG turnover.

Published
1988
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5. Exogenous glycosaminoglycans modulate extracellular and intracellular accumulation of newly synthesized glycosaminoglycans by embryonic chick skin fibroblasts.

Author

Evangelisti R, Stabellini G, Becchetti E, and Carinci P

Subjects
Animals, Chick Embryo, Chondroitin Sulfates metabolism, Dermatan Sulfate metabolism, Extracellular Space metabolism, Glycosaminoglycans metabolism, Heparin metabolism, Hyaluronic Acid metabolism, Skin metabolism, Fibroblasts metabolism, Glycosaminoglycans pharmacology, Skin cytology
Abstract

The effects of exogenous glycosaminoglycans (GAG) on newly synthesized GAG accumulation in intra- and extra-cellular compartments of 17 incubation days chick embryonic skin fibroblasts have been examined. Exogenous GAG are able to act on both total GAG synthesis and secretion by embryonic fibroblasts and on the relative concentration of individual GAG. A decline in hyaluronic acid and an increase in chondroitin sulfate plus dermatan sulfate in the cellular compartment for all GAG administered have been detected. There was also similar behaviour in the extracellular compartment after sulfated GAG administration.

Published
1989
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