1. Chemical genomics reveals targetable programs of human cancers rooted in pluripotency.
- Author
-
Orlando, Luca, Benoit, Yannick D., Reid, Jennifer C., Nakanishi, Mio, Boyd, Allison L., García-Rodriguez, Juan L., Tanasijevic, Borko, Doyle, Meaghan S., Luchman, Artee, Restall, Ian J., Bergin, Christopher J., Masibag, Angelique N., Aslostovar, Lili, Di Lu, Justin, Laronde, Sarah, Collins, Tony J., Weiss, Samuel, and Bhatia, Mickie
- Subjects
- *
EPIBLAST , *TUMOR suppressor genes , *PLURIPOTENT stem cells , *HUMAN stem cells , *GENOMICS , *DRUG side effects - Abstract
Overlapping principles of embryonic and tumor biology have been described, with recent multi-omics campaigns uncovering shared molecular profiles between human pluripotent stem cells (hPSCs) and adult tumors. Here, using a chemical genomic approach, we provide biological evidence that early germ layer fate decisions of hPSCs reveal targets of human cancers. Single-cell deconstruction of hPSCs-defined subsets that share transcriptional patterns with transformed adult tissues. Chemical screening using a unique germ layer specification assay for hPSCs identified drugs that enriched for compounds that selectively suppressed the growth of patient-derived tumors corresponding exclusively to their germ layer origin. Transcriptional response of hPSCs to germ layer inducing drugs could be used to identify targets capable of regulating hPSC specification as well as inhibiting adult tumors. Our study demonstrates properties of adult tumors converge with hPSCs drug induced differentiation in a germ layer specific manner, thereby expanding our understanding of cancer stemness and pluripotency. [Display omitted] • Subsets of hPSCs share molecular properties with cancers in a tissue specific manner • Barrier genes that block specific germ layer fates of hPSCs promote pluripotency • Drugs specifying hPSC germ layer fate suppress cancers of the same germ layer alone • Applied chemical genomics of hPSCs specification reveals targets of adult cancers Orlando et al. demonstrate that transcriptional features of pluripotency are shared with tissue specific human cancers that are organized by their germ layer origin, providing a foundation to understand properties of adult tumors related to cancer stemness. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF