Search

Your search keyword '"Krämer, Alwin"' showing total 10 results
Start Over Author "Krämer, Alwin" Journal cell cycle
10 results on '"Krämer, Alwin"'

1. Lack of centrioles and primary cilia inSTIL−/−mouse embryos

Author

David, Ahuvit, primary, Liu, Fengying, additional, Tibelius, Alexandra, additional, Vulprecht, Julia, additional, Wald, Diana, additional, Rothermel, Ulrike, additional, Ohana, Reut, additional, Seitel, Alexander, additional, Metzger, Jasmin, additional, Ashery-Padan, Ruth, additional, Meinzer, Hans-Peter, additional, Gröne, Hermann-Josef, additional, Izraeli, Shai, additional, and Krämer, Alwin, additional

Published
2014
Full Text
View/download PDF

9. Lack of centrioles and primary cilia in STIL−/−mouse embryos

Author

David, Ahuvit, Liu, Fengying, Tibelius, Alexandra, Vulprecht, Julia, Wald, Diana, Rothermel, Ulrike, Ohana, Reut, Seitel, Alexander, Metzger, Jasmin, Ashery-Padan, Ruth, Meinzer, Hans-Peter, Gröne, Hermann-Josef, Izraeli, Shai, and Krämer, Alwin

Abstract

Although most animal cells contain centrosomes, consisting of a pair of centrioles, their precise contribution to cell division and embryonic development is unclear. Genetic ablation of STIL, an essential component of the centriole replication machinery in mammalian cells, causes embryonic lethality in mice around mid gestation associated with defective Hedgehog signaling. Here, we describe, by focused ion beam scanning electron microscopy, that STIL−/−mouse embryos do not contain centrioles or primary cilia, suggesting that these organelles are not essential for mammalian development until mid gestation. We further show that the lack of primary cilia explains the absence of Hedgehog signaling in STIL−/−cells. Exogenous re-expression of STIL or STIL microcephaly mutants compatible with human survival, induced non-templated, de novogeneration of centrioles in STIL−/−cells. Thus, while the abscence of centrioles is compatible with mammalian gastrulation, lack of centrioles and primary cilia impairs Hedgehog signaling and further embryonic development.

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results