1. Targeting circular RNA-ZRANB1 for therapeutic intervention in retinal neurodegeneration
- Author
-
Rui Dong, Xiu-Miao Li, Rong-Mei Zhou, Jihong Wu, Biao Yan, Jia-Jian Wang, Shu-Jie Zhang, Shenghai Zhang, Bai-Hui Liu, Chang Liu, and Kun Shan
- Subjects
0301 basic medicine ,Male ,Cancer Research ,genetic structures ,Immunology ,Ependymoglial Cells ,Glaucoma ,Core Binding Factor Alpha 1 Subunit ,Biology ,Article ,Retina ,Cell Line ,Rats, Sprague-Dawley ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Downregulation and upregulation ,Circular RNA ,medicine ,Animals ,lcsh:QH573-671 ,Gene knockdown ,lcsh:Cytology ,Neurodegeneration ,Retinal ,Neurodegenerative Diseases ,Cell Biology ,RNA, Circular ,medicine.disease ,eye diseases ,Cell biology ,Rats ,Up-Regulation ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Retinal ganglion cell ,chemistry ,RNA ,sense organs ,Cell activation - Abstract
Glaucoma is a neurodegenerative disease characterized by retinal ganglion cell (RGC) loss, optic disc excavation, and progressive visual field loss. Direct or indirect ameliorating retinal neurodegeneration is a promising therapeutic therapy for glaucoma. Circular RNAs (circRNAs) are a class of covalently closed circular RNA transcripts and have emerged as potential regulators in several neurodegenerative diseases. In this study, we show that cZRANB1 expression is significantly upregulated in retinal neurodegeneration induced by glaucoma. cZRANB1 knockdown decreases retinal reactive gliosis, glial cell activation, and facilitates RGC survival in vivo. cZRANB1 knockdown directly regulates Müller cell function and indirectly regulates RGC function in vitro. cZRANB1 acts as miRNA sponge to regulate Müller cell function through cZRANB1/miR-217/RUNX2 network. Intervention of cZRANB1 expression would become an effective strategy for treating retinal neurodegeneration.
- Published
- 2018