1. POTEE drives colorectal cancer development via regulating SPHK1/p65 signaling
- Author
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Haijun Deng, Yuan Fang, Zhenkang Li, Yongsheng Li, Mingdao Lin, Yi Ding, Xiaochuang Feng, Yizhi Zhan, Zhiyong Shen, and Guoxin Li
- Subjects
Male ,Cancer Research ,Colorectal cancer ,Carcinogenesis ,Immunology ,Predictive markers ,Disease-Free Survival ,Article ,Cellular and Molecular Neuroscience ,eIF-2 Kinase ,Downregulation and upregulation ,Antigens, Neoplasm ,Cell Movement ,Cell Line, Tumor ,medicine ,Humans ,lcsh:QH573-671 ,Aged ,Regulation of gene expression ,Oncogene ,Cell growth ,Microarray analysis techniques ,business.industry ,lcsh:Cytology ,Cell Biology ,Middle Aged ,medicine.disease ,Gene Expression Regulation, Neoplastic ,Phosphotransferases (Alcohol Group Acceptor) ,Lymphatic Metastasis ,Cancer research ,Biomarker (medicine) ,Phosphorylation ,Female ,business ,Colorectal Neoplasms ,Signal Transduction - Abstract
Aberrant gene expression plays critical roles in the development of colorectal cancer (CRC). Here we show that POTEE, which was identified as a member E of POTE ankyrin domain family, was significantly upregulated in colorectal tumors and predicted poor overall survival of CRC patients. In CRC cells, POTEE could act as an oncogene and could promote cell growth, cell-cycle progression, inhibit apoptosis, and elevates xenograft tumor growth. Mechanically, we used microarray analysis and identified a POTEE/SPHK1/p65 signaling axis, which affected the biological functions of CRC cells. Further evaluation showed that overexpression of POTEE could increase the protein expression of SPHK1, followed by promoting the phosphorylation and activation of p65 protein. Altogether, our findings suggested a POTEE/SPHK1/p65 signaling axis could promote colorectal tumorigenesis and POTEE might potentially serve as a novel biomarker for the diagnosis and an intervention of colorectal cancer.
- Published
- 2019