1. Emergence and Transmission of Arbovirus Evolutionary Intermediates with Epidemic Potential
- Author
-
Kenneth A. Stapleford, Sreyrath Lay, Veasna Duong, Isabelle Bonne, Camilo Arias-Goeta, Christine Schmitt, Hervé Blanc, Anna-Bella Failloux, Stéphanie Beaucourt, Kathryn Rozen-Gagnon, Lark L. Coffey, Turkan Haliloglu, Ofer Isakov, Noam Shomron, Marco Vignuzzi, Antonio V. Bordería, Philippe Buchy, Nir Ben-Tal, Populations virales et Pathogenèse - Viral Populations and Pathogenesis, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), University of California [Davis] (UC Davis), University of California (UC), Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Sackler Faculty of Medicine, Tel Aviv University (TAU), Arbovirus et Insectes Vecteurs - Arboviruses and Insect Vectors, Institut Pasteur [Paris] (IP), Boǧaziçi üniversitesi = Boğaziçi University [Istanbul], Microscopie ultrastructurale - Ultrapole (CITECH), This work was supported by the European Research Council (ERC Starting Grant No. 242719), by the Bill and Melinda Gates Foundation Grand Challenges Exploration Initiative, the French Government’s Investissement d’Avenir program, Laboratoire d’Excellence 'Integrative Biology of Emerging Infectious Diseases' (grant ANR-10-LABX-62-IBEID), and the Israel-France High Council for Science and Technology Research 'Complexity in Biology' program. Salary for L.L.C. and K.A.S. was provided by the Region of Ile-de-France DIM program on Infectious, Parasitic or Nosocomial Emerging Diseases and the Philippe Foundation. Salary for A.V.B. was supported by the French National grant ANR-09-JCJC-0118-1. Salary for C.A.-G. was supported by the French Ministry of Superior Education and Research. N.B.-T. and T.H. were supported by NATO traveling grant CBP.MD.CLG 984340., We are grateful to Ngan Chantha, Huy Rekol, and the team of the National Malaria Centre in Cambodia for providing clinical specimens. We thank Christophe Paupy (IRD), Louis Lambrechts, and Paul Reiter (IP) for providing some mosquito strains., ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-09-JCJC-0118,QuasispeciesVax(2009), European Project: 242719,EC:FP7:ERC,ERC-2009-StG,RNAVIRUSPOPDIVNVAX(2009), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), University of California, Tel Aviv University [Tel Aviv], Institut Pasteur [Paris], and Boğaziçi University [Istanbul]
- Subjects
Cancer Research ,Mutation rate ,viruses ,MESH: Chikungunya Fever ,medicine.disease_cause ,Virus Replication ,Aedes ,MESH: Arboviruses ,MESH: Animals ,MESH: Genetic Variation ,Chikungunya ,Epidemic strain ,Genetics ,Mammals ,Transmission (medicine) ,virus diseases ,MESH: Aedes ,Viral Load ,Biological Evolution ,3. Good health ,[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology ,Host-Pathogen Interactions ,MESH: Arbovirus Infections ,Female ,MESH: Viral Load ,Cambodia ,Chikungunya virus ,MESH: Biological Evolution ,MESH: Insect Vectors ,Biology ,Arbovirus Infections ,Microbiology ,Arbovirus ,MESH: Mammals ,Virus ,Article ,MESH: Mice, Inbred C57BL ,Immunology and Microbiology(all) ,Virology ,parasitic diseases ,medicine ,Animals ,Humans ,MESH: Saliva ,Epidemics ,Saliva ,Molecular Biology ,MESH: Epidemics ,MESH: Humans ,MESH: Cambodia ,MESH: Host-Pathogen Interactions ,MESH: Virus Replication ,fungi ,RNA ,Genetic Variation ,MESH: Chikungunya virus ,medicine.disease ,Insect Vectors ,Mice, Inbred C57BL ,Disease Models, Animal ,Population bottleneck ,Culicidae ,Chikungunya Fever ,Parasitology ,MESH: Disease Models, Animal ,MESH: Culicidae ,MESH: Female ,Arboviruses - Abstract
Comment in: "Toward an activist agenda for monitoring virus emergence."https://doi.org/10.1016/j.chom.2014.05.014; International audience; The high replication and mutation rates of RNA viruses can result in the emergence of new epidemic variants. Thus, the ability to follow host-specific evolutionary trajectories of viruses is essential to predict and prevent epidemics. By studying the spatial and temporal evolution of chikungunya virus during natural transmission between mosquitoes and mammals, we have identified viral evolutionary intermediates prior to emergence. Analysis of virus populations at anatomical barriers revealed that the mosquito midgut and salivary gland pose population bottlenecks. By focusing on virus subpopulations in the saliva of multiple mosquito strains, we recapit-ulated the emergence of a recent epidemic strain of chikungunya and identified E1 glycoprotein mutations with potential to emerge in the future. These mutations confer fitness advantages in mosquito and mammalian hosts by altering virion stability and fusogenic activity. Thus, virus evolutionary tra-jectories can be predicted and studied in the short term before new variants displace currently circulating strains.
- Full Text
- View/download PDF