1. A protocol for generating induced T cells by reprogramming B cells in vivo
- Author
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Xiaofei Liu, Qitong Weng, Ying Wang, Mengyun Zhang, Cui Lv, Fangxiao Hu, Jinyong Wang, and Yong Dong
- Subjects
FITC, Fluorescein isothiocyanate ,0301 basic medicine ,Hoxb5 ,DAPI, 4,6-diamidino-2-phenylindole ,APC, Allophycocyanin ,T cells ,SP, single positive ,PB, peripheral blood ,Regenerative medicine ,Article ,law.invention ,iPSC, induced pluripotent stem cells ,03 medical and health sciences ,DN, double negative ,Plasmid ,Cell transplantation ,Retrovirus ,Phenotypic analysis ,In vivo ,law ,CAR-T, Chimeric antigen receptor T-Cell Immunotherapy ,lcsh:QH301-705.5 ,GFP, green fluorescent protein ,lcsh:R5-920 ,PerCP, Peridinin Chlorophyll ,biology ,Chemistry ,LN, Lymph node ,PE, Phycoerythrin ,Cell Biology ,biology.organism_classification ,HSC, Hematopoietic stem cells ,7-AAD, 7-Aminoactinomycin D ,BV, Brilliant Violet ,Cell biology ,TAA-TCR-T, tumor-associated antigen-TCR-T ,030104 developmental biology ,lcsh:Biology (General) ,Recombinant DNA ,lcsh:Medicine (General) ,Pro/pre-B cells ,Reprogramming ,Developmental Biology - Abstract
Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research. Keywords: Hoxb5, Retrovirus, Pro/pre-B cells, T cells
- Published
- 2018
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