1. Intradermal-delivered DNA vaccine induces durable immunity mediating a reduction in viral load in a rhesus macaque SARS-CoV-2 challenge model
- Author
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Mohamed Abdel-Mohsen, Laurent Humeau, Stephanie Ramos, J. Joseph Kim, Arthur Doan, Edgar Tello-Ruiz, Mansi Purwar, Anthony L. Cook, Faraz I. Zaidi, Brad Finneyfrock, Diana Guimet, Yaya Dia, Kate E. Broderick, Dustin Elwood, Alan Dodson, Viviane M Andrade, Laurent Pessaint, Jewell Walters, Ami Patel, Elizabeth Parzych, Neethu Chokkalingam, Jihae Choi, Ziyang Xu, Emma L. Reuschel, Igor Maricic, Trevor R.F. Smith, Zeena Eblimit, Opeyemi S. Adeniji, Kevin Kim, Ebony N. Gary, Sophia M. Reeder, Mark G. Lewis, John Harrison, Daniel W. Kulp, Susanne Walker, Alison Generotti, David B. Weiner, Karuppiah Muthumani, Nicholas J. Tursi, Hanne Leth Andersen, Katherine Schultheis, and Pratik Bhojnagarwala
- Subjects
Male ,electroporation ,Medicine (General) ,COVID-19 Vaccines ,Injections, Intradermal ,viruses ,T-Lymphocytes ,infectious disease ,coronavirus ,Antibodies, Viral ,General Biochemistry, Genetics and Molecular Biology ,Article ,DNA vaccination ,Immune system ,R5-920 ,Immunity ,Vaccines, DNA ,Medicine ,Animals ,Neutralizing antibody ,Lung ,biology ,business.industry ,SARS-CoV-2 ,Immunogenicity ,macaque ,COVID-19 ,Viral Load ,Acquired immune system ,protection ,Antibodies, Neutralizing ,Macaca mulatta ,ID DNA vaccine ,Vaccination ,Immunology ,Spike Glycoprotein, Coronavirus ,biology.protein ,challenge ,Female ,business ,Viral load - Abstract
Coronavirus disease 2019 (COVID-19), caused by the SARS-CoV-2 virus, has had a dramatic global impact on public health and social and economic infrastructures. Here, we assess the immunogenicity and anamnestic protective efficacy in rhesus macaques of an intradermal (i.d.)-delivered SARS-CoV-2 spike DNA vaccine, INO-4800, currently being evaluated in clinical trials. Vaccination with INO-4800 induced T cell responses and induced spike antigen and RBD binding antibodies with ADCP and ADCD activity. Sera from the animals neutralized both the D614 and G614 SARS-CoV-2 pseudotype viruses. Several months after vaccination, animals were challenged with SARS-CoV-2 resulting in rapid recall of anti-SARS-CoV-2 spike protein T cell and neutralizing antibody responses. These responses were associated with lower viral loads in the lung. These studies support the immune impact of INO-4800 for inducing both humoral and cellular arms of the adaptive immune system, which are likely important for providing durable protection against COVID-19 disease., Graphical abstract, Patel et al. demonstrate that immunization with a DNA vaccine encoding the SARS-CoV-2 spike antigen, INO-4800, induces durable immune responses in rhesus macaques and is associated with reduced viral loads after challenge.
- Published
- 2020