1. Human fetal mesenchymal stem cells differentiate into brown and white adipocytes: a role for ERRalpha in human UCP1 expression.
- Author
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Morganstein DL, Wu P, Mane MR, Fisk NM, White R, and Parker MG
- Subjects
- Adipocytes, Brown cytology, Adipocytes, White cytology, Animals, Calcium-Binding Proteins, Cell Differentiation, DNA-Binding Proteins genetics, DNA-Binding Proteins metabolism, Fatty Acid-Binding Proteins genetics, Fatty Acid-Binding Proteins metabolism, Fetus, Humans, Intercellular Signaling Peptides and Proteins genetics, Intercellular Signaling Peptides and Proteins metabolism, Membrane Proteins genetics, Membrane Proteins metabolism, Mice, PPAR gamma genetics, PPAR gamma metabolism, Receptors, Estrogen physiology, Transcription Factors genetics, Transcription Factors metabolism, Uncoupling Protein 1, ERRalpha Estrogen-Related Receptor, Adipocytes, Brown metabolism, Adipocytes, White metabolism, Ion Channels metabolism, Mesenchymal Stem Cells cytology, Mitochondrial Proteins metabolism, Receptors, Estrogen metabolism
- Abstract
We investigated the ability of fetal mesenchymal stem cells (fMSCs) to differentiate into brown and white adipocytes and compared the expression of a number of marker genes and key regulatory factors. We showed that the expression of key adipocyte regulators and markers during differentiation is similar to that in other human and murine adipocyte models, including induction of PPARgamma2 and FABP4. Notably, we found that the preadipocyte marker, Pref-1, is induced early in differentiation and then declines markedly as the process continues, suggesting that fMSCs first acquire preadipocyte characteristics as they commit to the adipogenic lineage, prior to their differentiation into mature adipocytes. After adipogenic induction, some stem cell isolates differentiated into cells resembling brown adipocytes and others into white adipocytes. Detailed investigation of one isolate showed that the novel brown fat-determining factor PRDM16 is expressed both before and after differentiation. Importantly, these cells exhibited elevated basal UCP-1 expression, which was dependent on the activity of the orphan nuclear receptor ERRalpha, highlighting a novel role for ERRalpha in human brown fat. Thus fMSCs represent a useful in vitro model for human adipogenesis, and provide opportunities to study the stages prior to commitment to the adipocyte lineage. They also offer invaluable insights into the characteristics of human brown fat.
- Published
- 2010
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