1. Reprogramming of Mouse and Human Cells to Pluripotency Using Mature MicroRNAs
- Author
-
Yuichiro Doki, Mitsugu Sekimoto, Koshi Mimori, Shinpei Nishikawa, Hirofumi Yamamoto, Dyah Laksmi Dewi, Ichiro Takemasa, Hideshi Ishii, Naotsugu Haraguchi, Masataka Ikeda, Masaki Mori, Fumiaki Tanaka, Yoshihiro Kano, Hiroaki Nagano, Toshiyuki Saito, Masahiro Tanemura, Norikatsu Miyoshi, Tsunekazu Mizushima, and Junichi Nishimura
- Subjects
Genetics ,Somatic cell ,Cellular differentiation ,fungi ,Cell Biology ,Transfection ,Biology ,Regenerative medicine ,Cell biology ,SOX2 ,KLF4 ,Molecular Medicine ,Induced pluripotent stem cell ,Reprogramming - Abstract
SummaryInduced pluripotent stem cells (iPSCs) can be generated from differentiated human and mouse somatic cells using transcription factors such as Oct4, Sox2, Klf4, and c-Myc. It is possible to augment the reprogramming process with chemical compounds, but issues related to low reprogramming efficiencies and, with a number of protocols, residual vector sequences, remain to be resolved. We show here that it is possible to reprogram mouse and human cells to pluripotency by direct transfection of mature double-stranded microRNAs (miRNAs). Our approaches use a combination of mir-200c plus mir-302 s and mir-369 s family miRNAs. Because this reprogramming method does not require vector-based gene transfer, it holds significant potential for biomedical research and regenerative medicine.
- Published
- 2011
- Full Text
- View/download PDF