1. Temporal Dynamics of VEGFA-Induced VEGFR2/FAK Co-Localization Depend on SHB
- Author
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Ilkka, Pietilä, Djenolan, Van Mourik, Andreas, Tamelander, Vitezslav, Kriz, Lena, Claesson-Welsh, Anders, Tengholm, and Michael, Welsh
- Subjects
Male ,Vascular Endothelial Growth Factor A ,SHB ,endocrine system ,Neovascularization, Physiologic ,TIRF ,Article ,Mice ,angiogenesis ,Cell Movement ,Proto-Oncogene Proteins ,Animals ,Humans ,Adaptor Proteins, Signal Transducing ,Mice, Knockout ,Mice, Inbred BALB C ,FAK ,Endothelial Cells ,respiratory system ,focal adhesions ,Vascular Endothelial Growth Factor Receptor-2 ,HEK293 Cells ,VEGFR2 ,Focal Adhesion Protein-Tyrosine Kinases ,cardiovascular system ,Female - Abstract
Focal adhesion kinase (FAK) is essential for vascular endothelial growth factor-A (VEGFA)/VEGF receptor-2 (VEGFR2)-stimulated angiogenesis and vascular permeability. We have previously noted that presence of the Src homology-2 domain adapter protein B (SHB) is of relevance for VEGFA-stimulated angiogenesis in a FAK-dependent manner. The current study was conducted in order address the temporal dynamics of co-localization between these components in HEK293 and primary lung endothelial cells (EC) by total internal reflection fluorescence microscopy (TIRF). An early (
- Published
- 2019