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In January of 2011, the Biomedical Engineering Society (BMES) and the Society for Physical Regulation in Biology and Medicine (SPRBM) held its inaugural Cellular and Molecular Bioengineering (CMBE) conference. The CMBE conference assembled worldwide leaders in the field of CMBE and held a very successful Round Table discussion among leaders. One of the action items was to collectively construct a white paper regarding the future of CMBE. Thus, the goal of this report is to emphasize the impact of CMBE as an emerging field, identify critical gaps in research that may be answered by the expertise of CMBE, and provide perspectives on enabling CMBE to address challenges in improving human health. Our goal is to provide constructive guidelines in shaping the future of CMBE.

In this review, we summarize the research progress in understanding the physiology of bone cells interacting with different mechanical/physical environments during bone tissue regeneration/repair. We first introduce the cellular composition of the bone tissue and the mechanism of the physiological bone regeneration/repair process. We then describe the properties and development of biomaterials for bone tissue engineering, followed by the highlighting of research progresses on the cellular response to mechanical environmental cues. Finally, several latest advancements in bone tissue regeneration and remaining challenges in the field are discussed for future research directions.

The Src/FAK complex is involved in many signaling pathways and plays crucial roles in cell adhesion/migration. It becomes clear that the subcellular localization of Src and FAK is crucial for their activities and functions. In this article, we first overview the molecular mechanisms and functions of Src and FAK involved in cell adhesion/migration. We then introduce the development of genetically encoded biosensors based on fluorescence resonance energy transfer (FRET) to visualize the activities of Src and FAK in live cells with high spatiotemporal resolutions. Different kinds of signal peptides targeting subcellular compartments are also discussed. FRET-based biosensors fused with these targeting signals peptides are further introduced to provide an overview on how these targeting signals can facilitate the localization of biosensors to continuously monitor the local activity of Src and FAK at subcellular compartments. In summary, genetically-encoded FRET biosensors integrated with subcellular compartment-targeting signals can provide powerful tools for the visualization of subcellular Src and FAK activities in live cells and advance our in-depth understanding of Src/FAK functions at different subcellular compartments.

Professor Shu Chien is a world-renowned leader and founder of Bioengineering. In particular, he has made seminal contributions to advancing our systematic and insightful understanding of how cells perceive their physical/mechanical environment and coordinate cellular functions. In this review, as part of a tribute to Prof. Shu Chien's scientific achievement, we summarize the research progress in understanding the physiology of bone cells interacting with different mechanical/physical environments during bone tissue regeneration/repair. We first introduce the cellular composition of the bone tissue and the mechanism of the physiological bone regeneration/repair process. We then describe the properties and development of biomaterials for bone tissue engineering, followed by the highlighting of research progresses on the cellular response to mechanical environmental cues. Finally, several latest advancements in bone tissue regeneration and remaining challenges in the field are discussed for future research directions.