1. Recent progress in whole-body radioautography
- Author
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M, Shimada and M, Watanabe
- Subjects
Male ,Paper ,Mice, Inbred ICR ,Neurotransmitter Agents ,Tissue Embedding ,Taurine ,Mice, Mutant Strains ,Receptor, Insulin ,Specimen Handling ,Macaca fascicularis ,Mice ,Glucose ,Pregnancy ,Glucose-6-Phosphatase ,Animals ,Autoradiography ,Frozen Sections ,Female ,Cysteine ,Cryoultramicrotomy - Abstract
We review whole-body radioautography for water-soluble substances and its applications studied to date in our laboratory. To transfer the whole-body section onto a glass slide, Japanese paper was inserted between frozen block and adhesive tape before sectioning. Then the section was dried together with the paper and adhesive tape in a cryotome and applied onto the glass slide coated with a mixture of egg-albumin and glycerin. The whole-body section was transferred to slide and contacted with the film in vacuum. By using these techniques, we review the glucose and taurine metabolism, the glucose-6-phosphatase activity and the insulin receptor.
- Published
- 1995