1. gamma-radiation-induced gammaH2AX formation occurs preferentially in actively transcribing euchromatic loci
- Author
-
Tom C. Karagiannis, Assam El-Osta, and Raja S. Vasireddy
- Subjects
Euchromatin ,DNA Repair ,Transcription, Genetic ,DNA repair ,DNA damage ,Heterochromatin ,Biology ,Epigenesis, Genetic ,Histones ,Cellular and Molecular Neuroscience ,Cell Line, Tumor ,Histone methylation ,Humans ,Molecular Biology ,Epigenesis ,Pharmacology ,Genetics ,Cell Biology ,DNA ,Cell biology ,Chromatin ,Nuclear DNA ,Gamma Rays ,Molecular Medicine ,DNA Damage - Abstract
The central dogma in radiation biology is that nuclear DNA is the critical target with respect to radiosensitivity. In accordance with the theoretical expectations, and in the absence of a conclusive model, the general consensus in the field has been to view chromatin as a homogeneous template for DNA damage and repair. This paradigm has been called into question by recent findings indicating a disparity in gamma-irradiation-induced gammaH2AX foci formation in euchromatin and heterochromatin. Here, we have extended those studies and provide evidence that gammaH2AX foci form preferentially in actively transcribing euchromatin following gamma-irradiation.
- Published
- 2009