1. Genetic loss of NFAT2 (NFATc1) impairs B cell development of B1 and B2 B cells.
- Author
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Märklin M, Heitmann JS, Kauer J, Wirths S, and Müller MR
- Subjects
- Animals, Antigens, Differentiation, B-Lymphocyte analysis, B-Lymphocyte Subsets metabolism, Female, Gene Expression Regulation, Developmental, Gene Knockout Techniques, Genes, Lethal, Heterozygote, Immunoglobulin D biosynthesis, Immunoglobulin D genetics, Immunoglobulin M biosynthesis, Immunoglobulin M genetics, Leukocyte Common Antigens biosynthesis, Leukocyte Common Antigens genetics, Lymphocyte Activation, Lymphoid Tissue growth & development, Lymphoid Tissue pathology, Lymphopoiesis genetics, Male, Mice, Mice, Inbred C57BL, NFATC Transcription Factors physiology, Organ Specificity, Specific Pathogen-Free Organisms, B-Lymphocyte Subsets cytology, Lymphopoiesis physiology, NFATC Transcription Factors deficiency
- Abstract
NFAT2 activity was shown to be of critical importance in B cell receptor signaling, development and proliferation; however its role in B cell development in the periphery is still not completely understood. We confirmed that NFAT2 deletion leads to impaired B1 B cell development, supported by our finding of limited B1 progenitors in the bone marrow and spleen of NFAT2 deficient mice. Moreover, we show for the first time that loss of NFAT2 increases immature B cells in particular transitional T2 and T3 as well as mature follicular B cells while marginal zone B cells are decreased. We further demonstrate that NFAT2 regulates the expression of B220, CD23, CD38, IgM/IgD and ZAP70 in murine B cells. In vivo analyses revealed decreased proliferation and increased apoptosis of NFAT2 deficient B cells. In summary, this study provides an extensive analysis of the role of NFAT2 in peripheral B lymphocyte development., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2020
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