1. Polymyxins and Analogues Bind to Ribosomal RNA and Interfere with Eukaryotic Translation in Vitro
- Author
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McCoy, Lisa S, Roberts, Kade D, Nation, Roger L, Thompson, Philip E, Velkov, Tony, Li, Jian, and Tor, Yitzhak
- Subjects
Microbiology ,Biological Sciences ,Infectious Diseases ,Anti-Bacterial Agents ,Bacteria ,Binding Sites ,Colistin ,Fluorescence Resonance Energy Transfer ,Fluorescent Dyes ,Polymyxin B ,Polymyxins ,Protein Biosynthesis ,RNA ,Ribosomal ,antibiotics ,colistin ,polymyxins ,RNA ,translation ,Medicinal and Biomolecular Chemistry ,Biochemistry and Cell Biology ,Organic Chemistry ,Biochemistry and cell biology ,Medicinal and biomolecular chemistry - Abstract
Looking for targets: while the bactericidal activity of polymyxins is attributed to changes in membrane permeation, we show that these antibiotics can bind prokaryotic and eukaryotic A-sites, domains responsible for translational decoding. Polymyxin B, colistin and analogues also hinder eukaryotic translation in vitro. These new targets and effects might be partially responsible for the plethora of adverse effects by these potent bactericidal agents.
- Published
- 2013