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Your search keyword '"*CELL imaging"' showing total 2 results
2 results on '"*CELL imaging"'

1. An activatable near-infrared fluorescent probe facilitated high-contrast lipophagic imaging in live cells.

Author

Chen, Wen, Luo, Na, Zhang, Yuan, Tang, Li-Juan, Wang, Fenglin, and Jiang, Jian-Hui

Subjects
*FLUORESCENT probes, *CELL imaging, *XANTHENE, *FLOW cytometry, *FLUORESCENCE
Abstract

A new fluorescent probe (Q-lipo) was developed by conjugating a xanthene scaffold with a quinoline moiety for activatable imaging of lipophagy. Q-lipo with acidic pH activated near infrared fluorescence and the lipid droplet targeting ability allowed activatable fluorescence imaging and flow cytometry detection of lipophagy in live cells with high contrast. It was further utilized to study the effect of tumor-microenvironment related conditions on lipophagy. Q-lipo would provide a useful tool for studying lipophagy in live cells. [ABSTRACT FROM AUTHOR]

Published
2021
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2. A flow cytometer-based whole cell screening toolbox for directed hydrolase evolution through fluorescent hydrogels.

Author

Lülsdorf, Nina, Pitzler, Christian, Biggel, Michael, Martinez, Ronny, Vojcic, Ljubica, and Schwaneberg, Ulrich

Subjects
*CELL imaging, *HYDROGELS, *FLOW cytometry, *HYDROLASES, *FLUORESCENCE
Abstract

A high throughput whole cell flow cytometer screening toolbox was developed and validated by identifying improved variants (1.3–7-fold) for three hydrolases (esterase, lipase, cellulase). The screening principle is based on coupled enzymatic reaction using glucose derivatives which yield upon hydrolysis a fluorescent-hydrogel-layer on the surface of E. coli cells. [ABSTRACT FROM AUTHOR]

Published
2015
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