1. High-resolution mapping and recognition of lipid domains using AFM with toxin-derivatized probes
- Author
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Maria Veiga-da-Cunha, Donatienne Tyteca, Cristina Lo Giudice, Andra C. Dumitru, David Alsteens, Sylvie Derclaye, Louise Conrard, and Patrick Henriet
- Subjects
0301 basic medicine ,Clostridium perfringens ,Membrane lipids ,Bacterial Toxins ,Lipid Bilayers ,Microscopy, Atomic Force ,Catalysis ,Hemolysin Proteins ,03 medical and health sciences ,Membrane Microdomains ,Molecular recognition ,Elastic Modulus ,Microscopy ,Materials Chemistry ,Lipid bilayer ,Toxins, Biological ,Atomic force microscopy ,Chemistry ,technology, industry, and agriculture ,Metals and Alloys ,General Chemistry ,Peptide Fragments ,Sphingomyelins ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Cholesterol ,030104 developmental biology ,Membrane ,Phosphatidylcholines ,Ceramics and Composites ,Biophysics ,Surface modification ,lipids (amino acids, peptides, and proteins) ,Sphingomyelin - Abstract
Cellular membrane lateral organization, in particular the assembly of lipids in domains, is difficult to evaluate at high resolution. Here, we used atomic force microscopy (AFM) to investigate at high-resolution lipid membranes containing variable amounts of sphingomyelin (SM) and cholesterol (Chol), two abundant membrane lipids. To this end, we developed new AFM tip functionalization strategies to specifically probe SM and Chol. Multiparametric AFM imaging allowed us to highlight the lateral submicrometric organization of these two lipids within lipid bilayers through the simultaneous topographic evidence of different phase regimes together with the extraction of their nanomechanical properties and the specific detection of lipid moieties by functionalized AFM probes. The combination of AFM topography and nanomechanical mapping with specific probes for molecular recognition of lipids represents a novel approach to identify lipid-enriched domains in supported bilayers and offers a unique perspective to directly observe lipid assemblies in living cells.
- Published
- 2018