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Start Over Author "Orada Chumphukam" Journal chemical communications
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1. Generation of a pair of independently binding DNA aptamers in a single round of selection using proximity ligation

Author

Thao T. Le, Anthony E. G. Cass, Sergey A. Piletsky, and Orada Chumphukam

Subjects
Chemistry, Aptamer, SELEX Aptamer Technique, Metals and Alloys, Nanotechnology, General Chemistry, Plasma protein binding, Aptamers, Nucleotide, DNA Aptamers, Polymerase Chain Reaction, Affinities, Catalysis, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Materials Chemistry, Ceramics and Composites, Biophysics, Nucleic Acid Conformation, Muramidase, Genomic library, Ligation, Selection (genetic algorithm), Gene Library, Protein Binding
Abstract

The ability to rapidly generate a pair of aptamers that bind independently to a protein target would greatly extend their use as reagents for two site ('sandwich') assays. We describe here a method to achieve this through proximity ligation. Using lysozyme as a target we demonstrate that under optimal conditions such a pair of aptamers, with nanomolar affinities, can be generated in a single round.

Published
2015
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