1. Two-dimensional LNA/DNA arrays: estimating the helicity of LNA/DNA hybrid duplex
- Author
-
Yan Liu, Hao Yan, and Sherri Rinker
- Subjects
Base pair ,Molecular Sequence Data ,Oligonucleotides ,Microscopy, Atomic Force ,Nucleic Acid Denaturation ,Catalysis ,chemistry.chemical_compound ,Nucleic acid thermodynamics ,Materials Chemistry ,A-DNA ,Locked nucleic acid ,Oligonucleotide Array Sequence Analysis ,Physics ,Base Sequence ,Oligonucleotide ,Metals and Alloys ,Temperature ,Nucleic Acid Hybridization ,General Chemistry ,DNA ,Oligonucleotides, Antisense ,Molecular biology ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Crystallography ,chemistry ,Ceramics and Composites ,Nucleic acid - Abstract
We measured the helical repeats of a non-natural nucleic acid, locked nucleic acid (LNA), by incorporating LNA strands into the outer arms of a DNA double crossover (DX) molecule; atomic force microscopy (AFM) imaging of the two-dimensional (2D) arrays self-assembled from these DX molecules allows us to derive the helical repeat of the LNA/DNA hetero-duplex to be 13.2 +/- 0.9 base pairs per turn.
- Published
- 2006