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1. Squaraine as a bright, stable and environment-sensitive far-red label for receptor-specific cellular imaging.

Author

Karpenko J, Klymchenko AS, Gioria S, Kreder R, Shulov I, Villa P, Mély Y, Hibert M, and Bonnet D

Subjects
Animals, Cattle, Color, HEK293 Cells, Humans, Protein Transport, Cyclobutanes chemistry, Fluorescent Dyes chemistry, Molecular Imaging, Phenols chemistry, Receptors, Oxytocin chemistry, Receptors, Oxytocin metabolism
Abstract

Herein, we show that a far-red arylidene-squaraine dye is stable against nucleophiles, in contrast to arene-squaraines. Owing to the fluorescence enhancement in apolar media together with high brightness and photostability, this dye was successfully applied to detect the oxytocin G protein-coupled receptor and monitor its internalization in living cells.

Published
2015
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