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1. Thioglycosynthases: double mutant glycosidases that serve as scaffolds for thioglycoside synthesis.

Author

Jahn M, Chen H, Müllegger J, Marles J, Warren RA, and Withers SG

Subjects
Circular Dichroism, Enzyme Stability, Glycoside Hydrolases chemistry, Hydrogen-Ion Concentration, Molecular Structure, Temperature, Thioglycosides chemistry, Glycoside Hydrolases genetics, Glycoside Hydrolases metabolism, Mutation genetics, Thioglycosides biosynthesis
Abstract

A double mutant, retaining glycosidase that lacks both the catalytic nucleophile and the catalytic acid/base residues efficiently catalyzes thioglycoside formation from a glycosyl fluoride donor and thiosugar acceptors.

Published
2004
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