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Start Over Author "Megan M. Wheeler" Journal chemical communications cambridge england

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1. Synthesis of selenomethylene-locked nucleic acid (SeLNA)-modified oligonucleotides by polymerases.

Author

Wheeler M, Chardon A, Goubet A, Morihiro K, Tsan SY, Edwards SL, Kodama T, Obika S, and Veedu RN

Subjects
Base Sequence, Molecular Sequence Data, Molecular Structure, Oligonucleotides chemistry, Polymerase Chain Reaction, DNA-Directed DNA Polymerase metabolism, Oligonucleotides chemical synthesis, Selenium chemistry
Abstract

Enzymatic recognition of SeLNA nucleotides was investigated. KOD XL DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of SeLNA-modified DNA templates was also efficiently achieved by Phusion and KOD XL DNA polymerases.

Published
2012
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