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Your search keyword '"Wombacher R"' showing total 2 results
Start Over Author "Wombacher R" Journal chemical communications cambridge england
2 results on '"Wombacher R"'

1. Aptamer-based proximity labeling guides covalent RNA modification.

Author

Englert D, Matveeva R, Sunbul M, Wombacher R, and Jäschke A

Subjects
Aptamers, Nucleotide chemical synthesis, Cycloaddition Reaction, Molecular Structure, Aptamers, Nucleotide chemistry, RNA chemistry, Staining and Labeling
Abstract

We describe the development of a proximity-induced bio-orthogonal inverse electron demand Diels-Alder reaction that exploits the high-affinity interaction between a dienophile-modified RhoBAST aptamer and its tetramethyl rhodamine methyltetrazine substrate. We applied this concept for covalent RNA labeling in proof-of-principle experiments.

Published
2021
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2. Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag.

Author

Gatzogiannis E, Chen Z, Wei L, Wombacher R, Kao YT, Yefremov G, Cornish VW, and Min W

Subjects
Carbocyanines analysis, Carbocyanines metabolism, Cell Survival, Escherichia coli genetics, Fluorescent Dyes analysis, HEK293 Cells, Histones analysis, Histones genetics, Histones metabolism, Humans, Recombinant Fusion Proteins analysis, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Tetrahydrofolate Dehydrogenase analysis, Tetrahydrofolate Dehydrogenase metabolism, Transfection, Trimethoprim analysis, Viscosity, Escherichia coli enzymology, Fluorescent Dyes metabolism, Microscopy, Fluorescence methods, Tetrahydrofolate Dehydrogenase genetics, Trimethoprim metabolism
Abstract

The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.

Published
2012
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