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• The non-edible Sinapis arvensis oil was considered for the synthesis of biofuel. • This study highlights the importance of lowering the reactant wastage. • Maximizing the overall efficiency in biofuel production is studied. • The physicochemical properties of Sinapis arvensis oil and biofuel is examined. • Sinapis arvensis biofuel could be a better substitute for existing fossil fuels. Large-scale biofuel production by utilizing edible crops leads to economic imbalance and depletion of food supply. Additionally, biofuel production costs from edible sources increase with time. Hence, biofuels derived from inedible feedstocks are the viable solution to these issues. The present research emphasizes reducing reactant wastage and optimizing overall efficiency in bio-oil synthesis from Sinapis Arvensis seed oil (SASO) to obtain the highest yield. The complete procedure will be carried out while considering optimized entities to increase methyl ester efficiency. The recommended optimal entities were discovered to be effective in maximizing yield. The Free Fatty Acids value of SASO is determined by employing isopropyl alcohol to study its potential as an alternative fuel. Oil was converted to crude biodiesel in a two-step transesterification process employing acid and base catalyst. An optimization approach was used to attain a high output and high biodiesel quality with lower waste. The Taguchi approach was used to optimize all of the process entities considered for this experiment. The most efficient and productive entities were identified using this approach. [ABSTRACT FROM AUTHOR]