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• Ab-oriented amorphous Zn-based nanocomposites are produced for nanolabels. • A powerful CAbD strategy is raised by innovatively integrating three elements. • A mLFIA mediated by CAbD strategy was constructed for monitoring of SEMI and AOZ. • The CAbD strategy can be a universal and scatheless mAb 1 orientation approach. • The CAbD strategy reduces mAb 1 consumption and enhances the sensitivity of mLFIA. The quantity and orientation of antibodies are key elements affecting the bioactivity of immunoprobes, which have important influences on the sensitivity and specificity of immuno-biosensors. In this work, a multiplex lateral flow immunoassay (mLFIA) mediated by a powerful cascaded antibody directionality (CAbD) strategy was constructed for the simultaneous monitoring of nitrofurazone and furazolidone metabolites in food samples. Here the CAbD strategy innovatively integrated three elements: antibody staining by Coomassie brilliant blue R-250, directional anchoring of second antibodies (Ab 2) by the amorphous ZnO-based nanocomposites (ZnO-CBB-Ab 2 , ZnO-NCMs), and orientation of detection antibodies (mAb 1) by the Ab 2. The ZnO-NCMs play a double role in antibody orientation and signaling to exhibit. Thanks to the difference in surface electrical properties of Fc and Fab segments in immunoglobulin G, the excessive R-250 stained Ab 2 was anchored in ZnO-NCMs to form a multi-detection signal tag with colorimetric signal brightness. Compared with immobilization strategies of directly directional anchor-hold or post-adsorption of mAb 1 , this strategy can reduce the mAb 1 consumption, better ensure the activity and utilization efficiency of mAb 1 , and shorten the construction time of the mLFIA system. As expected, the mLFIA is given satisfactory performances with a calculated sensitivity of 0.106 and 0.060 ng mL−1 for nitrofurazone and furazolidone metabolites, respectively. The developed multiplex immunosensor ultimately shows its application potential for point-of-care testing by performing well in fish and honey samples with accredited recoveries. It's worth emphasizing that the CAbD strategy can be a universal and scatheless antibody-directed approach, especially in multiplex immunoassays for ultrasensitive food safety monitoring. [ABSTRACT FROM AUTHOR]

A novel mesoporous graphitic carbon nitride/titanium dioxide (mpg-C 3 N 4 /TiO 2 ) nanocomposite was successfully synthesized and incorporated into polysulfone (PSf) matrix to fabricate photocatalytic membranes. This study aimed to explore the photocatalytic ability of the novel nanomaterial membrane in degrading the antibiotic sulfamethoxazole (SMX) under solar light. The structural and morphological properties of the mpg-C 3 N 4 /TiO 2 nanocomposite and membrane were characterized using various techniques. The SMX photocatalytic degradation performance, pathway and mechanism by mpg-C 3 N 4 /TiO 2 photocatalytic membrane reactor (PMR) were systematically investigated using HPLC and LC-MS/MS. As a pharmaceutically active compound, SMX was transformed into 7 kinds of non-toxic and pharmaceutically inactive byproducts by the innovative PMR technology. SMX removal efficiency of the membrane PSf-3 (with 1% mpg-C 3 N 4 /TiO 2 loading) was the highest over the 30 h consecutive irradiation. Meantime, the membrane didn’t affect the SMX photodegradation, and the structure was able to provide stable support with high integrity and flexibility after solar irradiation. The developed membrane has a great potential to be applied in water treatment industry. [ABSTRACT FROM AUTHOR]

[Display omitted] • Biomimetic injectable hydrogel embedded with Au/MOF composites was synthesized. • Au nanoparticles could improve visible light-driven photocatalysis of ZIF-8. • The hydrogel kills bacteria under visible light while promoting wound healing. Antibiotic resistance of bacteria is one of the greatest threats to wound healing, which necessitates the need for alternative strategies to eradicate bacteria in the wound site. Taking advantage of the reactive oxygen species (ROS)-generating capability of photocatalytic semiconductor nanomaterials under light irradiation, here we report a biomimetic injectable double-network hydrogel using oxidized sodium alginate and carbohydrazide-modified methacrylated gelatin that mimics the extracellular matrix, which was further embedded with semiconductor-like metal–organic frameworks (MOFs) encapsulating noble metal nanoparticles (Au@ZIF-8). This composite design substantially improved the ROS generation under visible light actuation (>400 nm) compared with pristine ZIF-8, owing to its reinforced light absorption and charge carrier separation by the Au-mediated surface plasmon resonance (SPR) and Schottky junction. The composite hydrogels not only showed remarkable bactericidal activity against both E. coli and S. aureus , but also significantly accelerated wound healing at optimal safety. Altogether, this injectable double-network hydrogel could simultaneously provide antibacterial and pro-healing capabilities, which may have translational potential as wound dressing materials. [ABSTRACT FROM AUTHOR]