1. Improving the reproducibility of size distribution of protein crystals produced in continuous slug flow crystallizer operated at short residence time
- Author
-
Kunn Hadinoto, Siyu Pu, and School of Chemical and Biomedical Engineering
- Subjects
Supersaturation ,Protein Crystallization ,Continuous Crystallization ,Materials science ,Yield (engineering) ,Economies of agglomeration ,Applied Mathematics ,General Chemical Engineering ,Nucleation ,02 engineering and technology ,General Chemistry ,021001 nanoscience & nanotechnology ,Residence time distribution ,Residence time (fluid dynamics) ,Slug flow ,Industrial and Manufacturing Engineering ,020401 chemical engineering ,Chemical engineering ,Chemical technology [Engineering] ,0204 chemical engineering ,0210 nano-technology ,Protein crystallization - Abstract
Continuous crystallization of proteins at short residence time and high supersaturation level is attractive in terms of the space-time yield and production efficiency. Nevertheless, it is rarely pursued due to its less-than-desirable crystal size distribution (CSD) characterized by the abundance of small crystals due to high nucleation rate. The small crystals were prone to random agglomeration, resulting in poor crystals’ residence time distribution, hence low CSD's reproducibility. Herein we developed a segmented slug flow crystallizer (SFC) design operated at short residence time (
- Published
- 2021