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Kaempferia galanga L. is a plant with medicinal value and is commonly used in folk remedies in Vietnam. The present study aimed to discover the antimicrobial and antifungal activities of ethanol crude extract and other fractions from rhizomes of Kaempferia galanga L. The antimicrobial effect of Kaempferia galanga L. extracts against bacteria strains including Escherichia coli, Lactobacillus sp. and Staphylococcus aureus was examined using the disk agar diffusion method. The antifungal activity against Fusarium sp., Collectotrichum sp., and Aspergillus flavus was discovered using disk agar diffusion and food poisoning techniques. The results reported that ethanol crude extract showed an inhibition zone against the three strains of bacteria including Escherichia coli, Lactobacillus sp. and Staphylococcus aureus. Besides that, the results also mentioned that the ethyl acetate extract possessed antifungal activities against Fusarium sp., Collectotrichum sp. and Aspergillus flavus. These findings concluded that Kaempferia galanga L. was a potential plant containing natural antimicrobial compounds.