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Granitic rocks derived by sequential partial melting are not well-documented worldwide. This paper provides another new important case in southern Tibet. The Yardoi gneiss dome within the Southern Tibet Rift System (STRS) consists of at least four suites of Miocene high-Sr/Y granites, formed at 20.4 Ma, 18.9 Ma, 17.7 Ma, and 17.1 Ma, respectively. Geochemical data indicate that they are Na-rich and peraluminous granites with high Sr/Y and La/Yb ratios. These granites show relatively radiogenic 87Sr/86Sr(t) (0.7091–0.7149) and unradiogenic ε Nd (t) (−11.1 to −7.7) isotopic signatures. As crystallization ages become younger, they are characterized by decreases in ε Nd (t) and Sr/Y but increases in 87Sr/86Sr(t) and Rb/Sr. Such temporal trends could be explained by sequential partial melting of first the mafic lower crustal rocks and then progressively shallower metasedimentary rocks. These findings together with literature-derived data, suggest that from north to south along the STRS, as extension proceeded with a heat source from the upwelling of asthenospheric mantle, sequential partial melting produced different Miocene magmatic rocks. This provides new insight into the mechanisms for the generation of geochemical and isotopic variations in spatially confined but temporally progressed granitic rocks. • The granites are Na-rich peraluminous ones with high Sr/Y and La/Yb ratios. • The granites have lower 87Sr/86Sr(t) but higher ε Nd (t) than other Miocene ones. • As crystallization ages become younger, ε Nd (t) decreases, 87Sr/86Sr(t) increases. • Data suggest sequential partial melting of first mafic and then metapelite rocks. • The E–W extension was active at ∼20 Ma. [ABSTRACT FROM AUTHOR]