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In this study, we amplified aquaporin 1(AQP1) promoter sequence with polymerase chain reaction(PCR), then AQP1 promoter fragment and pGL2 basic vector were linked to create an artificial pGL2/AQP1 promoter re-porter system. A certain concentration of 17β-estradiol(E2) activated pGL2/AQP1p, which demonstrated the pGL2/AQP1p transcriptional system effective. The pGL2/AQP1 promoter reporter system was applied to evaluate the activate effect on AQP1 of different kinds of phytoestrogens. Dual luciferase reporter gene activity assay showed that a certain concentration phytoestrogens including daidzein and genistein can increase AQP1 promoter transcription activity. In addition, E2, daidzein and genistein can make AQP1 protein endoge- nous expression level increase and promote the function of water scretion. The result can guide the clinical application to treat the Sjogren’s syndrome and provide a necessary molecular tool for the subsequent drug screening.