1. Acetylcholinesterase-Inhibitory Activities of the Extracts from Sponges Collected in Mauritius Waters
- Author
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Avin Ramanjooloo, Daniel E. P. Marie, Rob W. M. Van Soest, Rashmee Surnam-Boodhun, and Girish Beedessee
- Subjects
Aché ,Bioengineering ,Pharmacology ,Inhibitory postsynaptic potential ,Biochemistry ,chemistry.chemical_compound ,Neurochemical ,Non-competitive inhibition ,Alzheimer Disease ,medicine ,Animals ,Humans ,Molecular Biology ,Chemistry ,General Chemistry ,General Medicine ,Acetylcholinesterase ,language.human_language ,Terpenoid ,Porifera ,Kinetics ,language ,Mauritius ,Molecular Medicine ,Cholinesterase Inhibitors ,Chromatography, Thin Layer ,Amphimedon ,Acetylcholine ,Protein Binding ,medicine.drug - Abstract
Patients diagnosed with Alzheimer's disease (AD) show a characteristic neurochemical deficit of acetylcholine, especially in the basal forebrains. The use of acetylcholinesterase (AChE) inhibitors to retard the hydrolysis of acetylcholine has been suggested as a promising strategy for AD treatment. In this study, we evaluated the acetylcholinesterase inhibitory (AChEI) activities of 134 extracts obtained from 45 species of marine sponges. Thin-layer chromatography (TLC) and microplate assays reveal potent acetylcholinsterase inhibitory activities of two AcOEt extracts from the sponges Pericharax heteroraphis and Amphimedon navalis PULITZER-FINALI. We further investigated the inhibitory kinetics of the extracts and found them to display mixed competitive/noncompetitive inhibition and associated their inhibitory activity partly to terpenoids. Acetylcholinesterase inhibitors from marine organisms have been rarely studied, and this study demonstrated the potential of marine sponges as a source of pharmaceutical leads against neurodegenerative diseases.
- Published
- 2013
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