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Your search keyword '"Niko Hildebrandt"' showing total 3 results
Start Over Author "Niko Hildebrandt" Journal chemistry of materials
3 results on '"Niko Hildebrandt"'

1. Lanthanide-Complex-Loaded Polymer Nanoparticles for Background-Free Single-Particle and Live-Cell Imaging

Author

Andrey S. Klymchenko, Loïc J. Charbonnière, Anne Runser, Hortense Bartenlian, Niko Hildebrandt, Marcelina Cardoso Dos Santos, Andreas Reisch, Aline Nonat, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Bioimagerie et Pathologies (LBP), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Département Sciences Analytiques et Interactions Ioniques et Biomoléculaires (DSA-IPHC), Institut Pluridisciplinaire Hubert Curien (IPHC), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE09-0007,supertrack,Nanoparticules polymériques fluorescentes ultrabrillantes et biocompatibles pour l'imagerie rapide intracellulaire à haute-résolution(2016), Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, NanoBioPhotonics (NANO), Département Biochimie, Biophysique et Biologie Structurale (B3S), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Institut de Biologie Intégrative de la Cellule (I2BC), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay-Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Saclay, Université de Strasbourg (UNISTRA)-Institut National de Physique Nucléaire et de Physique des Particules du CNRS (IN2P3)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11), Laboratoire de Biophotonique et Pharmacologie - UMR 7213 (LBP), Réseau nanophotonique et optique, and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)

Subjects
Lanthanide, General Chemical Engineering, [SDV]Life Sciences [q-bio], Nanoparticle, Nanotechnology, quantum dots, 02 engineering and technology, bright, in-vitro, 010402 general chemistry, 01 natural sciences, dyes, Live cell imaging, emission, Materials Chemistry, luminescence, Molecule, [CHIM.COOR]Chemical Sciences/Coordination chemistry, ComputingMilieux_MISCELLANEOUS, time-resolved fluorescence, chemistry.chemical_classification, General Chemistry, Polymer, tracking, 021001 nanoscience & nanotechnology, 0104 chemical sciences, agents, Autofluorescence, chemistry, Particle, photoluminescence, Time-resolved spectroscopy, 0210 nano-technology
Abstract

WOS:000471728200021; International audience; Imaging single molecules and nanoparticles in complex biological media is highly challenging notably due to autofluorescence of cells and tissues. Lanthanides and lanthanide complexes, with their particularly long luminescence lifetimes, offer the possibility to perform time-gated imaging and thus to strongly reduce the autofluorescence background. However, their very low brightness and photon flux have limited their use in single-molecule imaging. Here, we encapsulate high amounts of Europium complexes into poly(methyl methacrylate)-based particles of 10, 20, and 30 nm size. The resulting particles contain up to 5000 copies of the complex with a quantum yield of \textgreater= 0.2, resulting in a per particle brightness of up to 4 x 10(7) M-1 cm(-1). They can be imaged at the single-particle level using low illumination intensities (0.24 W cm(-2)) and low acquisition times (300 ms) and internalize well into living cells, where they can be monitored through time-gated imaging at illumination conditions compatible with living specimen. These Eu-complex-loaded nanoparticles can thus be applied for highly sensitive and autofluorescence-free imaging and have the potential to become very performant probes for fast intracellular tracking of single biomolecules.

Published
2019
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2. Three-Dimensional Solution-Phase Förster Resonance Energy Transfer Analysis of Nanomolar Quantum Dot Bioconjugates with Subnanometer Resolution

Author

Michael H. Stewart, K. David Wegner, Ramasis Goswami, Niko Hildebrandt, Frank Morgner, Kimihiro Susumu, Igor L. Medintz, Eunkeu Oh, and Publica

Subjects
Bioconjugation, Chemistry, General Chemical Engineering, Resolution (electron density), Nanotechnology, 02 engineering and technology, General Chemistry, 010402 general chemistry, 021001 nanoscience & nanotechnology, 7. Clean energy, 01 natural sciences, Solution phase, 0104 chemical sciences, Characterization (materials science), Förster resonance energy transfer, Quantum dot, Materials Chemistry, 0210 nano-technology, Luminescence, Biosensor
Abstract

Luminescent semiconductor quantum dots (QDs) play an important role in optical biosensing and, in particular, in FRET (Forster resonance energy transfer)-based luminescent probes. The QD materials that form the basis for these probes are in actuality quite heterogeneous and consist of different types of QDs with variations in material compositions, surface coatings, and available biofunctionalization strategies. To optimize their role in active sensors that rely on FRET, extensive physicochemical characterization is required. A technique that can provide precise information about size, shape, and bioconjugation properties of different QD-biomolecule conjugates from a single sample and measurement under actual experimental biosensing conditions would therefore be highly important for advancing QDs to a next generation nanobiosensing tool. Here, we present a detailed FRET study on a large set of QD-biomolecule conjugates, which allows for a homogeneous solution-phase size, shape, and bioconjugation analysis of peptide and protein self-assembled QDs at subnanomolar concentrations and with subnanometer resolution. Direct incorporation of luminescent Tb-complexes (Tb) in the peptides or proteins leads to Tb-to-QD FRET upon assembly to the different QD surfaces. Luminescence decay times and time-gated intensities, which precisely decode the FRET interactions, provide a wealth of useful information on the underlying composite structure and even biochemical functionality. In contrast to other high-resolution techniques, which require rather sophisticated instrumentation, well-defined experimental conditions, and low sample throughput, our technique uses a commercial time-resolved fluorescence plate reader for very fast and simple data acquisition of many aqueous samples in a standard microtiter plate.

Published
2014
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