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Synthesis of ultrafine Co1-(x+y)NixZnyFe2O4 magnetic nanoparticles with different chemical compositions is essential to study the magnetic fluid hyperthermia (MFH) as a treatment in which the superparamagnetic behaviour of materials is beneficial. In this paper, Co1-(x+y)NixZnyFe2O4 magnetic nanoparticles with different x and y amounts were synthesized to find out a tuning pattern for magnetic properties, especially anisotropy constant to gain the best heating efficiency. We proved that the co‐presence of ions will greatly affect the magnetic performance of our superparamagnetic nanoparticles in whichCo0.59Zn0.41Fe2O4 for values of x=0.00 and y=0.41 exhibits the highest magnetic anisotropy constant after CoFe2O4 CoFe2O4. This success in customizing magnetic properties to reach high magnetic properties by using less toxic materials might be another good starting point for researchers to focus more on their possible applications. [ABSTRACT FROM AUTHOR]